mouse monoclonal anti rab5  (Cell Signaling Technology Inc)

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation

doi: 10.1016/j.jcmgh.2022.10.008

Figure Lengend Snippet: Key Resources

Article Snippet: Mouse monoclonal Anti-RAB5 , Santa Cruz Biotechnology , Cat# sc-46692, RRID:AB_628191.

Techniques: Virus, Subcloning, Purification, Recombinant, Protease Inhibitor, In Situ, Fluorescence, Activation Assay, Enzyme-linked Immunosorbent Assay, Luciferase, Isolation, shRNA, Control, Plasmid Preparation, Software

Journal: Frontiers in Genetics

Article Title: Down Syndrome Fetal Fibroblasts Display Alterations of Endosomal Trafficking Possibly due to SYNJ1 Overexpression

doi: 10.3389/fgene.2022.867989

Figure Lengend Snippet: The dynamics of early endosomes is perturbed in DS-HFFs. (A) EU-HFFs and DS-HFFs were stained with Rab5 antibody detected with Alexa-546-conjugated secondary antibodies. Serial confocal sections were collected from the top to the bottom of the cells. For each condition, the 3D reconstruction and corresponding intensity map are shown. Scale bars, 8 μm. Mean fluorescence intensity (arbitrary unit, a.u.) of Rab5-positive structures is higher in DS-HFFs than in EU-HFFs. The bars show the relative mean value ± SD of three independent experiments. (B) Representative 3D reconstruction and corresponding intensity map of cell ROIs taken into analysis (left panels). Two populations of Rab5 endosomes are distinguishable in terms of brightness, with lower (arrows) and higher (arrowheads) intensity fluorescent signal. Representative scatter dot plot showing the distribution of fluorescence intensity of Rab5-positive structures in EU-HFFs and DS-HFFs (right panel). Each dot represents one sample value, error bars represent mean value ± SD. (C) The percentage of larger Rab5 positive structures (>1 μm) are increased in DS-HFFs when compared to that in EU-HFFs. The bars show the relative mean value ± SD of three independent experiments, n ≥ 30 cells. (D) Representative immunoblotting of Rab5 in DS-HFFs and EU-HFFs. GAPDH was used as the loading control. The molecular weight of protein markers is indicated. Densitometric analysis of three different experiments, performed in three euploid and in four trisomic cultures, is shown. Error bars, mean ± SD. * p < 0.05; *** p < 0.001, Student’s t -test.

Article Snippet: Primary antibodies included the following: rabbit polyclonal anti-Cathepsin D (Thermo Fisher Scientific), rabbit polyclonal anti-EEA1 (Thermo Fisher Scientific), rabbit polyclonal anti-FGF receptor 1 (Cell Signaling Technology), mouse monoclonal anti-Lamp-1 (BD Pharmingen™), mouse monoclonal anti-Rab5 (BD Transduction Laboratories™), mouse monoclonal anti-Rab7 (Santa Cruz Biotechnology, Inc.), and rabbit polyclonal anti-Synj1 (Abcam plc).

Techniques: Staining, Fluorescence, Western Blot, Molecular Weight

Journal: eLife

Article Title: The amyloid precursor protein is a conserved Wnt receptor

doi: 10.7554/eLife.69199

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-rab5 , Synaptic Systems , Cat# 108 011 RRID: AB_887773 , IF (1:100) WB (1:1000).

Techniques: Isolation, Plasmid Preparation, Sequencing, Transfection, Construct, Virus, Reverse Transcription, SYBR Green Assay, Software